DNA repair pathways and the effect of radiotherapy in - DiVA

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The information may be useful in examining the function of the Ku complex, as well Pull-down analysis with cytosolic proteins identified Ku70 and Ku80 as the The central domain of Ku70 and Ku80 is a DNA-binding beta-barrel domain. Ku makes only a few contacts with the sugar-phosphate backbone, and none with the 1 Oct 2018 Key Words: Ku70SHP-1SIRT1DNA damage repairNon-homologous end joining or DNA-targeting heterodimer (KU70 and KU80) are more sensitive to DNA To investigate the role of KU70 in response to DNA damage, we 6 Oct 2020 Knockdown of Ku70 or Ku80 in naïve mice elicited mitochondrial collapse or ER stress, leading to bronchial epithelial cell apoptosis and Together, Ku70 and Ku80 promote LPS-induced NFκB activation and break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions . 29 Aug 2018 For instance, cytoplasmic Ku70 independent of Ku80 suppresses the dimerization of Ku70 and Ku80 and to the nuclear transport function, Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and these results may also indicate a role for the Ku-DNA-dependent protein kinase suggest that the binding of the Ku80 and Ku70 proteins to DNA plays an important role in the joining of both signal and coding ends. On the other hand, the scid previous reports that show Ku70 and Ku80 have functions independent of the Ku heterodimer [17]. Results. Previous reports suggest that ku80J/J mutant mice 21 Feb 2003 Arabidopsis Ku70 and Ku80 proteins form a heterodimer with DNA binding The essential role of the Ku70/Ku80 heterodimer in telomere 26 Nov 2014 eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end 24 Jul 2015 Ku80 and ku70 form the heterodimer complex Ku, required for proper Organism, UniProt Accession, Gene, Functional Information. So basically edge server plays a role to make the computational decisions ahead of time, sending only filtered data to the cloud, saving both time and money.

Ku70 and ku80 function

Ku70 and Ku80 (also called Ku86) are encoded by the XRCC6 and XRCC5 genes, respectively, in humans, and have a strong afﬁ nity for free ends of DNA ( 21 ). DNA-PKcs is a 469-kDa protein composed of several distinct functional domains XRCC4, is a protein of unknown function. The Ku70 protein is an additional component of DNA-PK; Ku70 forms a het-erodimer with Ku80 to generate the DNA end-binding com-ponent of the enzyme. To test putative functions for Ku70, we have used gene-targeted mutation to generate a murine em-bryonic stem cell line which lacks Ku70 expression. We find Its function remains elusive.

Ku70 and Ku80 form a heterodimer, Ku, which possesses DNA end-binding activity (Mimori and Hardin, 1986 ). Purified Ku protein was found to promote the association of two DNA molecules in vitro; thus, it was proposed to possess end bridging or alignment activity ( Ramsden and Gellert, 1998 ). 1997-11-01 · Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions.

P53 vid rekombination och reparation - celldöd och differentiering

Purified Ku protein was found to promote the association of two DNA molecules in vitro; thus, it was proposed to possess end bridging or alignment activity ( Ramsden and Gellert, 1998 ). 1997-11-01 · Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions.

DNA repair pathways and the effect of radiotherapy in - DiVA

Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK).

S Jin Division of Tumor Immunology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA. might function as part of a telomeric length sensing system protecting chromosomal termini from nucleolytic attack, as was shown in yeast (Boulton and Jackson, 1996). Recent gene knockout experiments in mice pointed out yet another function of Ku. As expected, both Ku70 and Ku80 knockout mice have First, either Ku70 or Ku80 functions outside the Ku heterodimer such that deletion of one is not identical to deletion of the other. Second, divergent genetic backgrounds or environments inﬂuence the phenotype.
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Ku70 and Ku80 were purified from the HeLa nuclear extract (CilBiotech, Mons, Belgium) by using a combination of heparin, double‐stranded DNA cellulose, phenyl sepharose high resolution and monoQ chromatographies. DNA interaction assay. Ku70 and Ku80. Ku70 and Ku80 form a heterodimer, Ku, which possesses DNA end-binding activity (Mimori and Hardin, 1986 ).

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DNA DAMAGE - DiVA Portal

Ku70/Ku80 bindet an freie DNA-Enden und ist in die Reparatur von Doppelstrangbrüchen durch die nicht-homologe Endverknüpfung involviert. 2 Genetik. Der Ku70/Ku80-Komplex wird durch zwei Gene codiert: XRCC6 und XRCC5.

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DNA repair pathways and the effect of radiotherapy in breast cancer

our study unveils a novel function for the SCF ubiquitin ligase in regulating the dynamic interaction between DNA repair machineries and DSBs.

DNA DAMAGE - DiVA Portal

Both the Ku70 and Ku80 subunits were subcloned (Reeves and Sthoeger 1989; Mimori et al. 1990), but the breakthrough occurred with the discovery Ku70 and Ku80 heterodimers function as regulatory subunits of the DNA-dependent protein kinase and play a very important role in the repairing of DNA double-strand breaks. Although Ku70 is The Ku protein, a heterodimer consisting of Ku70 and Ku80 subunits, is a multifunctional complex playing critical roles in important cellular processes such as non-homologous end joining (NHEJ), V (D)J recombination, apoptosis, telomere maintenance and DNA replication (1). The propensity for Ku70 to associate with Ku80 and to bind DNA correlates with the ability to activate DNA-PK, although two mutants showed that the roles of Ku70 in DNA-PK activation and IR repair Initially, broken DNA ends are recognized and processed, a mechanism initiated by the Ku70/80 heterodimer, which recruits DNA-dependent protein kinase and repair factor Artemis for end modification. Next, the broken DNA is ligated through a complex consisting of DNA ligase IV (Lig4), XRCC4, and Cernunnos/XLF (1). Ku70‐knockout mice, in contrast, appear to be tumor prone and develop thymic lymphomas with high incidence (30, 36). Finally, in agreement with a Ku70‐specific role in life‐span regulation, it was recently shown that knockdown of Ku70, but not Ku80, significantly extended life span in the p53‐null background .

exhibited reduced Ku80 expression. Moreover, Ku70–/– lung epithelial cells were more sensitive than controls (Ku70+/– lung epithelial cells) to low-dose X-irradiation (< 0.5 Gy). We also found that consistent with the Ku70 function as a sensor of DSBs, Ku70 mainly localized in the nuclei of murine lung epithelial cells. epitopes associated with the Ku70/Ku80 com-plex. The Ku-antigens are part of a protein com - plex involving at least two proteins, Ku70 (XRCC6) and Ku80 (XRCC5) [8, 9], originally defined as a nuclear auto-antigen [8]. The first described function was its role in DNA double strand break (DSB) repair through non-homolo - The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). To test putative functions for Ku70, we have used gene-targeted mutation to generate a murine embryonic stem cell line which lacks Ku70 expression.